Unless specifically noted, all roles are open to all regardless of race or ethnicity.

• Jo March – Our story's protagonist. A passionate and determined young author struggling to find her place in the world. She has the idea of writing Little Women and eventually becomes engaged to Professor Bhaer.
• Amy March – The youngest, most energetic sister with a rather pompous air about her. She later marries Laurie. Doubles as the troll in the operatic tragedy.
• Meg March – The world-weary, yet hopeful, oldest sister who yearns for a great life. She marries John and has twins with him. Doubles as Clarissa in the operatic tragedy.
• Beth March – The second youngest of the sisters. Timid and musical, Beth encourages and helps her sisters selflessly. Forms an unlikely friendship with the crotchety Mr Laurence. Doubles as Rodrigo II in the operatic tragedy.
• Marmee March – The March girls' mother. She is the strong backbone of the family, who is courageous in spite of the difficult odds she faces. Doubles as the hag in the operatic tragedy.
• Aunt March – A formidable, over-bearing matron and great-aunt to the March sisters. The wealthy socialite in town.
• Theodore 'Laurie' Laurence – Lonely and charming boy next door who becomes firm friends with the March family. Proposes to Jo but eventually falls in love with and marries Amy. Doubles as Rodrigo in the operatic tragedy.
• John Brooke – Laurie's tutor. A reserved and hard-working young man who only shows his tender side when he falls in love with Meg. Doubles as Braxton Prendergast in the operatic tragedy.
• Professor Bhaer – A sensible German professor boarding with Mrs Kirk. Persuades Jo that she is better than the "blood and guts stuff" she writes. Falls in love with, and eventually becomes engaged to Jo.
• Mr. Laurence – Laurie's grandfather. A stiff and stern elderly man who eventually shows his softer side and becomes friends with Beth.
• Mrs. Kirk – The Irish owner of the boarding house in which Professor Bhaer and Jo meet.